Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
7.3% |
| Arg[R] |
60 |
7.4% |
| Asn[N] |
30 |
3.7% |
| Asp[D] |
62 |
7.6% |
| Cys[C] |
7 |
0.9% |
| Gln[Q] |
27 |
3.3% |
| Glu[E] |
66 |
8.1% |
| Gly[G] |
42 |
5.2% |
| His[H] |
11 |
1.4% |
| Ile[I] |
60 |
7.4% |
| Leu[L] |
92 |
11.3% |
| Lys[K] |
71 |
8.8% |
| Met[M] |
16 |
2.0% |
| Phe[F] |
20 |
2.5% |
| Pro[P] |
29 |
3.6% |
| Ser[S] |
46 |
5.7% |
| Thr[T] |
43 |
5.3% |
| Trp[W] |
2 |
0.2% |
| Tyr[Y] |
15 |
1.8% |
| Val[V] |
53 |
6.5% |
| Total Length |
811 |
|
| Instability index (II) |
32.91 |
| This classifies the protein as stable |
|
| Carbon |
4016 |
| Hydrogen |
6637 |
| Nitrogen |
1143 |
| Oxygen |
1229 |
| Sulfur |
23 |
| Total number of atoms |
13048 |
| Formula = C4016H6637N1143O1229S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
33725 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
33350 M-1 cm-1 |
| assuming all Cys residues are reduced |
33350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
33725 M-1 cm-1 |
|
|
| Codon Bias |
0.187 |
| Codon Adaptation Index |
0.207 |
| Frequency of Optimal Codons |
0.511 |
| Hydropathicity of Protein |
-0.405 |
| Aromaticity Score |
0.046 |
|
