Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
11 |
2.4% |
| Arg[R] |
13 |
2.8% |
| Asn[N] |
31 |
6.7% |
| Asp[D] |
29 |
6.3% |
| Cys[C] |
12 |
2.6% |
| Gln[Q] |
9 |
2.0% |
| Glu[E] |
27 |
5.9% |
| Gly[G] |
22 |
4.8% |
| His[H] |
15 |
3.3% |
| Ile[I] |
30 |
6.5% |
| Leu[L] |
44 |
9.5% |
| Lys[K] |
39 |
8.5% |
| Met[M] |
11 |
2.4% |
| Phe[F] |
19 |
4.1% |
| Pro[P] |
17 |
3.7% |
| Ser[S] |
56 |
12.1% |
| Thr[T] |
25 |
5.4% |
| Trp[W] |
2 |
0.4% |
| Tyr[Y] |
21 |
4.6% |
| Val[V] |
28 |
6.1% |
| Total Length |
461 |
|
| Instability index (II) |
47.69 |
| This classifies the protein as unstable |
|
| Carbon |
2309 |
| Hydrogen |
3623 |
| Nitrogen |
611 |
| Oxygen |
716 |
| Sulfur |
23 |
| Total number of atoms |
7282 |
| Formula = C2309H3623N611O716S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
43040 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
42290 M-1 cm-1 |
| assuming all Cys residues are reduced |
42290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
43040 M-1 cm-1 |
|
|
| Codon Bias |
0.096 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.387 |
| Aromaticity Score |
0.091 |
|
