Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
13 |
2.4% |
| Arg[R] |
16 |
2.9% |
| Asn[N] |
28 |
5.1% |
| Asp[D] |
26 |
4.8% |
| Cys[C] |
10 |
1.8% |
| Gln[Q] |
25 |
4.6% |
| Glu[E] |
40 |
7.4% |
| Gly[G] |
12 |
2.2% |
| His[H] |
7 |
1.3% |
| Ile[I] |
37 |
6.8% |
| Leu[L] |
75 |
13.8% |
| Lys[K] |
54 |
9.9% |
| Met[M] |
10 |
1.8% |
| Phe[F] |
30 |
5.5% |
| Pro[P] |
12 |
2.2% |
| Ser[S] |
49 |
9.0% |
| Thr[T] |
24 |
4.4% |
| Trp[W] |
13 |
2.4% |
| Tyr[Y] |
41 |
7.5% |
| Val[V] |
22 |
4.0% |
| Total Length |
544 |
|
| Instability index (II) |
41.12 |
| This classifies the protein as unstable |
|
| Carbon |
3013 |
| Hydrogen |
4606 |
| Nitrogen |
726 |
| Oxygen |
844 |
| Sulfur |
20 |
| Total number of atoms |
9209 |
| Formula = C3013H4606N726O844S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
133215 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
132590 M-1 cm-1 |
| assuming all Cys residues are reduced |
132590 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
133215 M-1 cm-1 |
|
|
| Codon Bias |
0.007 |
| Codon Adaptation Index |
0.131 |
| Frequency of Optimal Codons |
0.424 |
| Hydropathicity of Protein |
-0.315 |
| Aromaticity Score |
0.154 |
|
