Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
8.7% |
| Arg[R] |
20 |
2.9% |
| Asn[N] |
39 |
5.6% |
| Asp[D] |
33 |
4.8% |
| Cys[C] |
9 |
1.3% |
| Gln[Q] |
25 |
3.6% |
| Glu[E] |
41 |
5.9% |
| Gly[G] |
58 |
8.4% |
| His[H] |
16 |
2.3% |
| Ile[I] |
46 |
6.6% |
| Leu[L] |
62 |
8.9% |
| Lys[K] |
53 |
7.6% |
| Met[M] |
9 |
1.3% |
| Phe[F] |
19 |
2.7% |
| Pro[P] |
34 |
4.9% |
| Ser[S] |
50 |
7.2% |
| Thr[T] |
39 |
5.6% |
| Trp[W] |
11 |
1.6% |
| Tyr[Y] |
17 |
2.5% |
| Val[V] |
52 |
7.5% |
| Total Length |
693 |
|
| Instability index (II) |
39.43 |
| This classifies the protein as stable |
|
| Carbon |
3349 |
| Hydrogen |
5327 |
| Nitrogen |
913 |
| Oxygen |
1012 |
| Sulfur |
18 |
| Total number of atoms |
10619 |
| Formula = C3349H5327N913O1012S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
86330 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
85830 M-1 cm-1 |
| assuming all Cys residues are reduced |
85830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
86330 M-1 cm-1 |
|
|
| Codon Bias |
0.232 |
| Codon Adaptation Index |
0.200 |
| Frequency of Optimal Codons |
0.541 |
| Hydropathicity of Protein |
-0.211 |
| Aromaticity Score |
0.068 |
|
