Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
4.3% |
| Arg[R] |
48 |
3.5% |
| Asn[N] |
100 |
7.4% |
| Asp[D] |
77 |
5.7% |
| Cys[C] |
5 |
0.4% |
| Gln[Q] |
57 |
4.2% |
| Glu[E] |
108 |
8.0% |
| Gly[G] |
39 |
2.9% |
| His[H] |
26 |
1.9% |
| Ile[I] |
89 |
6.6% |
| Leu[L] |
92 |
6.8% |
| Lys[K] |
158 |
11.6% |
| Met[M] |
20 |
1.5% |
| Phe[F] |
37 |
2.7% |
| Pro[P] |
77 |
5.7% |
| Ser[S] |
174 |
12.8% |
| Thr[T] |
98 |
7.2% |
| Trp[W] |
6 |
0.4% |
| Tyr[Y] |
21 |
1.5% |
| Val[V] |
67 |
4.9% |
| Total Length |
1358 |
|
| Instability index (II) |
46.85 |
| This classifies the protein as unstable |
|
| Carbon |
6603 |
| Hydrogen |
10741 |
| Nitrogen |
1875 |
| Oxygen |
2179 |
| Sulfur |
25 |
| Total number of atoms |
21423 |
| Formula = C6603H10741N1875O2179S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
64540 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
64290 M-1 cm-1 |
| assuming all Cys residues are reduced |
64290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
64540 M-1 cm-1 |
|
|
| Codon Bias |
0.014 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.429 |
| Hydropathicity of Protein |
-0.884 |
| Aromaticity Score |
0.047 |
|
