Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
57 |
4.3% |
| Arg[R] |
61 |
4.6% |
| Asn[N] |
120 |
9.1% |
| Asp[D] |
86 |
6.5% |
| Cys[C] |
22 |
1.7% |
| Gln[Q] |
37 |
2.8% |
| Glu[E] |
75 |
5.7% |
| Gly[G] |
46 |
3.5% |
| His[H] |
37 |
2.8% |
| Ile[I] |
74 |
5.6% |
| Leu[L] |
147 |
11.1% |
| Lys[K] |
86 |
6.5% |
| Met[M] |
29 |
2.2% |
| Phe[F] |
75 |
5.7% |
| Pro[P] |
48 |
3.6% |
| Ser[S] |
151 |
11.4% |
| Thr[T] |
71 |
5.4% |
| Trp[W] |
4 |
0.3% |
| Tyr[Y] |
49 |
3.7% |
| Val[V] |
48 |
3.6% |
| Total Length |
1323 |
|
| Instability index (II) |
40.58 |
| This classifies the protein as unstable |
|
| Carbon |
6665 |
| Hydrogen |
10397 |
| Nitrogen |
1827 |
| Oxygen |
2074 |
| Sulfur |
51 |
| Total number of atoms |
21014 |
| Formula = C6665H10397N1827O2074S51 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
96385 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
95010 M-1 cm-1 |
| assuming all Cys residues are reduced |
95010 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
96385 M-1 cm-1 |
|
|
| Codon Bias |
0.047 |
| Codon Adaptation Index |
0.154 |
| Frequency of Optimal Codons |
0.435 |
| Hydropathicity of Protein |
-0.498 |
| Aromaticity Score |
0.097 |
|
