Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
44 |
6.1% |
| Arg[R] |
17 |
2.3% |
| Asn[N] |
37 |
5.1% |
| Asp[D] |
28 |
3.9% |
| Cys[C] |
7 |
1.0% |
| Gln[Q] |
16 |
2.2% |
| Glu[E] |
23 |
3.2% |
| Gly[G] |
32 |
4.4% |
| His[H] |
34 |
4.7% |
| Ile[I] |
61 |
8.4% |
| Leu[L] |
109 |
15.1% |
| Lys[K] |
34 |
4.7% |
| Met[M] |
12 |
1.7% |
| Phe[F] |
47 |
6.5% |
| Pro[P] |
27 |
3.7% |
| Ser[S] |
78 |
10.8% |
| Thr[T] |
51 |
7.0% |
| Trp[W] |
6 |
0.8% |
| Tyr[Y] |
20 |
2.8% |
| Val[V] |
41 |
5.7% |
| Total Length |
724 |
|
| Instability index (II) |
34.63 |
| This classifies the protein as stable |
|
| Carbon |
3709 |
| Hydrogen |
5800 |
| Nitrogen |
936 |
| Oxygen |
1029 |
| Sulfur |
19 |
| Total number of atoms |
11493 |
| Formula = C3709H5800N936O1029S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
63175 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62800 M-1 cm-1 |
| assuming all Cys residues are reduced |
62800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
63175 M-1 cm-1 |
|
|
| Codon Bias |
-0.010 |
| Codon Adaptation Index |
0.116 |
| Frequency of Optimal Codons |
0.394 |
| Hydropathicity of Protein |
0.338 |
| Aromaticity Score |
0.101 |
|
