SDH4/YDR178W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 6.6%
Arg[R] 6 3.3%
Asn[N] 5 2.8%
Asp[D] 6 3.3%
Cys[C] 2 1.1%
Gln[Q] 3 1.7%
Glu[E] 9 5.0%
Gly[G] 13 7.2%
His[H] 3 1.7%
Ile[I] 7 3.9%
Leu[L] 17 9.4%
Lys[K] 13 7.2%
Met[M] 10 5.5%
Phe[F] 9 5.0%
Pro[P] 11 6.1%
Ser[S] 16 8.8%
Thr[T] 13 7.2%
Trp[W] 3 1.7%
Tyr[Y] 10 5.5%
Val[V] 13 7.2%
Total Length 181

Instability index
Instability index (II) 49.18
This classifies the protein as unstable

Atomic composition
Carbon 922
Hydrogen 1427
Nitrogen 229
Oxygen 259
Sulfur 12
Total number of atoms 2849
Formula = C922H1427N229O259S12

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 31525 M-1 cm-1
assuming NO Cys residues appear as half cystines 31400 M-1 cm-1
assuming all Cys residues are reduced 31400 M-1 cm-1
assuming all pairs of Cys residues form cystines 31525 M-1 cm-1

Aliphatic index
Aliphatic index 79.17

Coding Region Translation Calculations
Codon Bias 0.217
Codon Adaptation Index 0.194
Frequency of Optimal Codons 0.524
Hydropathicity of Protein -0.037
Aromaticity Score 0.122