Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
14 |
6.5% |
| Arg[R] |
8 |
3.7% |
| Asn[N] |
10 |
4.7% |
| Asp[D] |
16 |
7.4% |
| Cys[C] |
1 |
0.5% |
| Gln[Q] |
8 |
3.7% |
| Glu[E] |
18 |
8.4% |
| Gly[G] |
11 |
5.1% |
| His[H] |
6 |
2.8% |
| Ile[I] |
15 |
7.0% |
| Leu[L] |
20 |
9.3% |
| Lys[K] |
16 |
7.4% |
| Met[M] |
4 |
1.9% |
| Phe[F] |
8 |
3.7% |
| Pro[P] |
14 |
6.5% |
| Ser[S] |
15 |
7.0% |
| Thr[T] |
10 |
4.7% |
| Trp[W] |
2 |
0.9% |
| Tyr[Y] |
6 |
2.8% |
| Val[V] |
13 |
6.0% |
| Total Length |
215 |
|
| Instability index (II) |
53.62 |
| This classifies the protein as unstable |
|
| Carbon |
1079 |
| Hydrogen |
1697 |
| Nitrogen |
287 |
| Oxygen |
333 |
| Sulfur |
5 |
| Total number of atoms |
3401 |
| Formula = C1079H1697N287O333S5 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
19940 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
19940 M-1 cm-1 |
| assuming all Cys residues are reduced |
19940 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
19940 M-1 cm-1 |
|
|
| Codon Bias |
0.094 |
| Codon Adaptation Index |
0.158 |
| Frequency of Optimal Codons |
0.464 |
| Hydropathicity of Protein |
-0.461 |
| Aromaticity Score |
0.074 |
|
