Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
4.8% |
| Arg[R] |
30 |
4.3% |
| Asn[N] |
56 |
8.0% |
| Asp[D] |
65 |
9.3% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
37 |
5.3% |
| Glu[E] |
60 |
8.5% |
| Gly[G] |
34 |
4.8% |
| His[H] |
6 |
0.9% |
| Ile[I] |
38 |
5.4% |
| Leu[L] |
62 |
8.8% |
| Lys[K] |
70 |
10.0% |
| Met[M] |
11 |
1.6% |
| Phe[F] |
21 |
3.0% |
| Pro[P] |
43 |
6.1% |
| Ser[S] |
52 |
7.4% |
| Thr[T] |
40 |
5.7% |
| Trp[W] |
5 |
0.7% |
| Tyr[Y] |
12 |
1.7% |
| Val[V] |
25 |
3.6% |
| Total Length |
702 |
|
| Instability index (II) |
42.42 |
| This classifies the protein as unstable |
|
| Carbon |
3441 |
| Hydrogen |
5510 |
| Nitrogen |
972 |
| Oxygen |
1150 |
| Sulfur |
12 |
| Total number of atoms |
11085 |
| Formula = C3441H5510N972O1150S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
45380 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
45380 M-1 cm-1 |
| assuming all Cys residues are reduced |
45380 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
45380 M-1 cm-1 |
|
|
| Codon Bias |
0.022 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.424 |
| Hydropathicity of Protein |
-1.008 |
| Aromaticity Score |
0.054 |
|
