Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
4.2% |
| Arg[R] |
16 |
4.5% |
| Asn[N] |
16 |
4.5% |
| Asp[D] |
43 |
12.1% |
| Cys[C] |
2 |
0.6% |
| Gln[Q] |
9 |
2.5% |
| Glu[E] |
28 |
7.9% |
| Gly[G] |
21 |
5.9% |
| His[H] |
5 |
1.4% |
| Ile[I] |
19 |
5.4% |
| Leu[L] |
41 |
11.5% |
| Lys[K] |
28 |
7.9% |
| Met[M] |
9 |
2.5% |
| Phe[F] |
14 |
3.9% |
| Pro[P] |
11 |
3.1% |
| Ser[S] |
32 |
9.0% |
| Thr[T] |
16 |
4.5% |
| Trp[W] |
2 |
0.6% |
| Tyr[Y] |
14 |
3.9% |
| Val[V] |
14 |
3.9% |
| Total Length |
355 |
|
| Instability index (II) |
40.65 |
| This classifies the protein as unstable |
|
| Carbon |
1772 |
| Hydrogen |
2780 |
| Nitrogen |
468 |
| Oxygen |
585 |
| Sulfur |
11 |
| Total number of atoms |
5616 |
| Formula = C1772H2780N468O585S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
31985 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
31860 M-1 cm-1 |
| assuming all Cys residues are reduced |
31860 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
31985 M-1 cm-1 |
|
|
| Codon Bias |
-0.074 |
| Codon Adaptation Index |
0.111 |
| Frequency of Optimal Codons |
0.360 |
| Hydropathicity of Protein |
-0.641 |
| Aromaticity Score |
0.085 |
|
