Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
6.3% |
| Arg[R] |
18 |
2.6% |
| Asn[N] |
45 |
6.6% |
| Asp[D] |
30 |
4.4% |
| Cys[C] |
5 |
0.7% |
| Gln[Q] |
53 |
7.7% |
| Glu[E] |
57 |
8.3% |
| Gly[G] |
60 |
8.8% |
| His[H] |
13 |
1.9% |
| Ile[I] |
32 |
4.7% |
| Leu[L] |
35 |
5.1% |
| Lys[K] |
66 |
9.6% |
| Met[M] |
19 |
2.8% |
| Phe[F] |
16 |
2.3% |
| Pro[P] |
30 |
4.4% |
| Ser[S] |
35 |
5.1% |
| Thr[T] |
39 |
5.7% |
| Trp[W] |
4 |
0.6% |
| Tyr[Y] |
35 |
5.1% |
| Val[V] |
50 |
7.3% |
| Total Length |
685 |
|
| Instability index (II) |
41.43 |
| This classifies the protein as unstable |
|
| Carbon |
3357 |
| Hydrogen |
5279 |
| Nitrogen |
933 |
| Oxygen |
1067 |
| Sulfur |
24 |
| Total number of atoms |
10660 |
| Formula = C3357H5279N933O1067S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74400 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
74150 M-1 cm-1 |
| assuming all Cys residues are reduced |
74150 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74400 M-1 cm-1 |
|
|
| Codon Bias |
0.359 |
| Codon Adaptation Index |
0.315 |
| Frequency of Optimal Codons |
0.630 |
| Hydropathicity of Protein |
-0.797 |
| Aromaticity Score |
0.080 |
|
