HSP42/YDR171W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 17 4.5%
Arg[R] 18 4.8%
Asn[N] 22 5.9%
Asp[D] 23 6.1%
Cys[C] 1 0.3%
Gln[Q] 20 5.3%
Glu[E] 43 11.5%
Gly[G] 24 6.4%
His[H] 12 3.2%
Ile[I] 15 4.0%
Leu[L] 24 6.4%
Lys[K] 25 6.7%
Met[M] 4 1.1%
Phe[F] 9 2.4%
Pro[P] 35 9.3%
Ser[S] 23 6.1%
Thr[T] 18 4.8%
Trp[W] 0 0.0%
Tyr[Y] 24 6.4%
Val[V] 18 4.8%
Total Length 375

Instability index
Instability index (II) 57.56
This classifies the protein as unstable

Atomic composition
Carbon 1884
Hydrogen 2882
Nitrogen 520
Oxygen 615
Sulfur 5
Total number of atoms 5906
Formula = C1884H2882N520O615S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 35760 M-1 cm-1
assuming NO Cys residues appear as half cystines 35760 M-1 cm-1
assuming all Cys residues are reduced 35760 M-1 cm-1
assuming all pairs of Cys residues form cystines 35760 M-1 cm-1

Aliphatic index
Aliphatic index 59.01

Coding Region Translation Calculations
Codon Bias 0.195
Codon Adaptation Index 0.182
Frequency of Optimal Codons 0.523
Hydropathicity of Protein -1.127
Aromaticity Score 0.088