Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
2.6% |
| Arg[R] |
33 |
3.4% |
| Asn[N] |
91 |
9.4% |
| Asp[D] |
51 |
5.3% |
| Cys[C] |
14 |
1.4% |
| Gln[Q] |
57 |
5.9% |
| Glu[E] |
67 |
6.9% |
| Gly[G] |
32 |
3.3% |
| His[H] |
11 |
1.1% |
| Ile[I] |
93 |
9.6% |
| Leu[L] |
109 |
11.2% |
| Lys[K] |
72 |
7.4% |
| Met[M] |
14 |
1.4% |
| Phe[F] |
52 |
5.4% |
| Pro[P] |
27 |
2.8% |
| Ser[S] |
95 |
9.8% |
| Thr[T] |
54 |
5.6% |
| Trp[W] |
10 |
1.0% |
| Tyr[Y] |
32 |
3.3% |
| Val[V] |
32 |
3.3% |
| Total Length |
971 |
|
| Instability index (II) |
44.25 |
| This classifies the protein as unstable |
|
| Carbon |
5009 |
| Hydrogen |
7890 |
| Nitrogen |
1322 |
| Oxygen |
1537 |
| Sulfur |
28 |
| Total number of atoms |
15786 |
| Formula = C5009H7890N1322O1537S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
103555 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
102680 M-1 cm-1 |
| assuming all Cys residues are reduced |
102680 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
103555 M-1 cm-1 |
|
|
| Codon Bias |
0.063 |
| Codon Adaptation Index |
0.160 |
| Frequency of Optimal Codons |
0.464 |
| Hydropathicity of Protein |
-0.408 |
| Aromaticity Score |
0.097 |
|
