Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
4.1% |
| Arg[R] |
30 |
4.6% |
| Asn[N] |
41 |
6.3% |
| Asp[D] |
52 |
8.0% |
| Cys[C] |
8 |
1.2% |
| Gln[Q] |
19 |
2.9% |
| Glu[E] |
39 |
6.0% |
| Gly[G] |
27 |
4.1% |
| His[H] |
12 |
1.8% |
| Ile[I] |
60 |
9.2% |
| Leu[L] |
72 |
11.0% |
| Lys[K] |
48 |
7.4% |
| Met[M] |
6 |
0.9% |
| Phe[F] |
36 |
5.5% |
| Pro[P] |
22 |
3.4% |
| Ser[S] |
51 |
7.8% |
| Thr[T] |
27 |
4.1% |
| Trp[W] |
6 |
0.9% |
| Tyr[Y] |
32 |
4.9% |
| Val[V] |
37 |
5.7% |
| Total Length |
652 |
|
| Instability index (II) |
35.54 |
| This classifies the protein as stable |
|
| Carbon |
3417 |
| Hydrogen |
5318 |
| Nitrogen |
880 |
| Oxygen |
1005 |
| Sulfur |
14 |
| Total number of atoms |
10634 |
| Formula = C3417H5318N880O1005S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
81180 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
80680 M-1 cm-1 |
| assuming all Cys residues are reduced |
80680 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
81180 M-1 cm-1 |
|
|
| Codon Bias |
-0.066 |
| Codon Adaptation Index |
0.114 |
| Frequency of Optimal Codons |
0.381 |
| Hydropathicity of Protein |
-0.248 |
| Aromaticity Score |
0.113 |
|
