Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
5.3% |
| Arg[R] |
31 |
4.4% |
| Asn[N] |
44 |
6.3% |
| Asp[D] |
44 |
6.3% |
| Cys[C] |
12 |
1.7% |
| Gln[Q] |
24 |
3.4% |
| Glu[E] |
46 |
6.6% |
| Gly[G] |
19 |
2.7% |
| His[H] |
17 |
2.4% |
| Ile[I] |
40 |
5.7% |
| Leu[L] |
81 |
11.6% |
| Lys[K] |
49 |
7.0% |
| Met[M] |
21 |
3.0% |
| Phe[F] |
37 |
5.3% |
| Pro[P] |
25 |
3.6% |
| Ser[S] |
68 |
9.7% |
| Thr[T] |
36 |
5.2% |
| Trp[W] |
9 |
1.3% |
| Tyr[Y] |
26 |
3.7% |
| Val[V] |
32 |
4.6% |
| Total Length |
698 |
|
| Instability index (II) |
40.43 |
| This classifies the protein as unstable |
|
| Carbon |
3599 |
| Hydrogen |
5613 |
| Nitrogen |
951 |
| Oxygen |
1077 |
| Sulfur |
33 |
| Total number of atoms |
11273 |
| Formula = C3599H5613N951O1077S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
88990 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
88240 M-1 cm-1 |
| assuming all Cys residues are reduced |
88240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
88990 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.127 |
| Frequency of Optimal Codons |
0.407 |
| Hydropathicity of Protein |
-0.351 |
| Aromaticity Score |
0.103 |
|
