Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
3.9% |
| Arg[R] |
41 |
4.5% |
| Asn[N] |
72 |
7.9% |
| Asp[D] |
63 |
6.9% |
| Cys[C] |
16 |
1.7% |
| Gln[Q] |
28 |
3.1% |
| Glu[E] |
56 |
6.1% |
| Gly[G] |
33 |
3.6% |
| His[H] |
20 |
2.2% |
| Ile[I] |
68 |
7.4% |
| Leu[L] |
93 |
10.1% |
| Lys[K] |
50 |
5.5% |
| Met[M] |
10 |
1.1% |
| Phe[F] |
41 |
4.5% |
| Pro[P] |
51 |
5.6% |
| Ser[S] |
112 |
12.2% |
| Thr[T] |
58 |
6.3% |
| Trp[W] |
6 |
0.7% |
| Tyr[Y] |
20 |
2.2% |
| Val[V] |
43 |
4.7% |
| Total Length |
917 |
|
| Instability index (II) |
48.01 |
| This classifies the protein as unstable |
|
| Carbon |
4517 |
| Hydrogen |
7136 |
| Nitrogen |
1236 |
| Oxygen |
1446 |
| Sulfur |
26 |
| Total number of atoms |
14361 |
| Formula = C4517H7136N1236O1446S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
63800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62800 M-1 cm-1 |
| assuming all Cys residues are reduced |
62800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
63800 M-1 cm-1 |
|
|
| Codon Bias |
0.035 |
| Codon Adaptation Index |
0.135 |
| Frequency of Optimal Codons |
0.430 |
| Hydropathicity of Protein |
-0.423 |
| Aromaticity Score |
0.073 |
|
