Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.9% |
| Arg[R] |
23 |
3.9% |
| Asn[N] |
23 |
3.9% |
| Asp[D] |
31 |
5.2% |
| Cys[C] |
9 |
1.5% |
| Gln[Q] |
27 |
4.6% |
| Glu[E] |
34 |
5.7% |
| Gly[G] |
38 |
6.4% |
| His[H] |
15 |
2.5% |
| Ile[I] |
38 |
6.4% |
| Leu[L] |
68 |
11.5% |
| Lys[K] |
40 |
6.7% |
| Met[M] |
9 |
1.5% |
| Phe[F] |
12 |
2.0% |
| Pro[P] |
27 |
4.6% |
| Ser[S] |
51 |
8.6% |
| Thr[T] |
52 |
8.8% |
| Trp[W] |
6 |
1.0% |
| Tyr[Y] |
15 |
2.5% |
| Val[V] |
40 |
6.7% |
| Total Length |
593 |
|
| Instability index (II) |
40.68 |
| This classifies the protein as unstable |
|
| Carbon |
2883 |
| Hydrogen |
4662 |
| Nitrogen |
788 |
| Oxygen |
892 |
| Sulfur |
18 |
| Total number of atoms |
9243 |
| Formula = C2883H4662N788O892S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
55850 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
55350 M-1 cm-1 |
| assuming all Cys residues are reduced |
55350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
55850 M-1 cm-1 |
|
|
| Codon Bias |
0.267 |
| Codon Adaptation Index |
0.238 |
| Frequency of Optimal Codons |
0.561 |
| Hydropathicity of Protein |
-0.231 |
| Aromaticity Score |
0.056 |
|
