Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
55 |
5.9% |
| Arg[R] |
31 |
3.4% |
| Asn[N] |
106 |
11.5% |
| Asp[D] |
50 |
5.4% |
| Cys[C] |
10 |
1.1% |
| Gln[Q] |
36 |
3.9% |
| Glu[E] |
52 |
5.6% |
| Gly[G] |
29 |
3.1% |
| His[H] |
19 |
2.1% |
| Ile[I] |
55 |
5.9% |
| Leu[L] |
86 |
9.3% |
| Lys[K] |
60 |
6.5% |
| Met[M] |
19 |
2.1% |
| Phe[F] |
31 |
3.4% |
| Pro[P] |
46 |
5.0% |
| Ser[S] |
111 |
12.0% |
| Thr[T] |
46 |
5.0% |
| Trp[W] |
16 |
1.7% |
| Tyr[Y] |
25 |
2.7% |
| Val[V] |
42 |
4.5% |
| Total Length |
925 |
|
| Instability index (II) |
43.61 |
| This classifies the protein as unstable |
|
| Carbon |
4555 |
| Hydrogen |
7140 |
| Nitrogen |
1274 |
| Oxygen |
1454 |
| Sulfur |
29 |
| Total number of atoms |
14452 |
| Formula = C4555H7140N1274O1454S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
125875 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125250 M-1 cm-1 |
| assuming all Cys residues are reduced |
125250 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
125875 M-1 cm-1 |
|
|
| Codon Bias |
-0.008 |
| Codon Adaptation Index |
0.130 |
| Frequency of Optimal Codons |
0.404 |
| Hydropathicity of Protein |
-0.588 |
| Aromaticity Score |
0.078 |
|
