Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
11 |
2.7% |
| Arg[R] |
24 |
5.9% |
| Asn[N] |
31 |
7.6% |
| Asp[D] |
24 |
5.9% |
| Cys[C] |
7 |
1.7% |
| Gln[Q] |
16 |
3.9% |
| Glu[E] |
29 |
7.1% |
| Gly[G] |
24 |
5.9% |
| His[H] |
6 |
1.5% |
| Ile[I] |
39 |
9.6% |
| Leu[L] |
36 |
8.9% |
| Lys[K] |
27 |
6.7% |
| Met[M] |
7 |
1.7% |
| Phe[F] |
25 |
6.2% |
| Pro[P] |
10 |
2.5% |
| Ser[S] |
38 |
9.4% |
| Thr[T] |
20 |
4.9% |
| Trp[W] |
3 |
0.7% |
| Tyr[Y] |
8 |
2.0% |
| Val[V] |
21 |
5.2% |
| Total Length |
406 |
|
| Instability index (II) |
52.15 |
| This classifies the protein as unstable |
|
| Carbon |
2053 |
| Hydrogen |
3259 |
| Nitrogen |
567 |
| Oxygen |
626 |
| Sulfur |
14 |
| Total number of atoms |
6519 |
| Formula = C2053H3259N567O626S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
28795 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
28420 M-1 cm-1 |
| assuming all Cys residues are reduced |
28420 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
28795 M-1 cm-1 |
|
|
| Codon Bias |
-0.030 |
| Codon Adaptation Index |
0.110 |
| Frequency of Optimal Codons |
0.404 |
| Hydropathicity of Protein |
-0.356 |
| Aromaticity Score |
0.089 |
|
