FMP16/YDR070C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 5.4%
Arg[R] 9 9.7%
Asn[N] 5 5.4%
Asp[D] 9 9.7%
Cys[C] 0 0.0%
Gln[Q] 5 5.4%
Glu[E] 6 6.5%
Gly[G] 5 5.4%
His[H] 2 2.2%
Ile[I] 2 2.2%
Leu[L] 8 8.6%
Lys[K] 11 11.8%
Met[M] 3 3.2%
Phe[F] 4 4.3%
Pro[P] 6 6.5%
Ser[S] 4 4.3%
Thr[T] 5 5.4%
Trp[W] 0 0.0%
Tyr[Y] 2 2.2%
Val[V] 2 2.2%
Total Length 93

Instability index
Instability index (II) 38.37
This classifies the protein as stable

Atomic composition
Carbon 469
Hydrogen 759
Nitrogen 145
Oxygen 145
Sulfur 3
Total number of atoms 1521
Formula = C469H759N145O145S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 53.55

Coding Region Translation Calculations
Codon Bias 0.309
Codon Adaptation Index 0.266
Frequency of Optimal Codons 0.578
Hydropathicity of Protein -1.339
Aromaticity Score 0.065