Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
28 |
5.2% |
| Arg[R] |
25 |
4.6% |
| Asn[N] |
31 |
5.8% |
| Asp[D] |
27 |
5.0% |
| Cys[C] |
9 |
1.7% |
| Gln[Q] |
16 |
3.0% |
| Glu[E] |
40 |
7.4% |
| Gly[G] |
32 |
5.9% |
| His[H] |
14 |
2.6% |
| Ile[I] |
39 |
7.2% |
| Leu[L] |
59 |
10.9% |
| Lys[K] |
39 |
7.2% |
| Met[M] |
15 |
2.8% |
| Phe[F] |
20 |
3.7% |
| Pro[P] |
27 |
5.0% |
| Ser[S] |
45 |
8.3% |
| Thr[T] |
16 |
3.0% |
| Trp[W] |
9 |
1.7% |
| Tyr[Y] |
18 |
3.3% |
| Val[V] |
30 |
5.6% |
| Total Length |
539 |
|
| Instability index (II) |
48.37 |
| This classifies the protein as unstable |
|
| Carbon |
2743 |
| Hydrogen |
4319 |
| Nitrogen |
737 |
| Oxygen |
800 |
| Sulfur |
24 |
| Total number of atoms |
8623 |
| Formula = C2743H4319N737O800S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
76820 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
76320 M-1 cm-1 |
| assuming all Cys residues are reduced |
76320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
76820 M-1 cm-1 |
|
|
| Codon Bias |
-0.022 |
| Codon Adaptation Index |
0.099 |
| Frequency of Optimal Codons |
0.388 |
| Hydropathicity of Protein |
-0.297 |
| Aromaticity Score |
0.087 |
|
