NRG1/YDR043C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 3.0%
Arg[R] 15 6.5%
Asn[N] 20 8.7%
Asp[D] 10 4.3%
Cys[C] 7 3.0%
Gln[Q] 13 5.6%
Glu[E] 15 6.5%
Gly[G] 9 3.9%
His[H] 9 3.9%
Ile[I] 9 3.9%
Leu[L] 17 7.4%
Lys[K] 17 7.4%
Met[M] 4 1.7%
Phe[F] 7 3.0%
Pro[P] 10 4.3%
Ser[S] 25 10.8%
Thr[T] 16 6.9%
Trp[W] 1 0.4%
Tyr[Y] 12 5.2%
Val[V] 8 3.5%
Total Length 231

Instability index
Instability index (II) 51.45
This classifies the protein as unstable

Atomic composition
Carbon 1153
Hydrogen 1807
Nitrogen 345
Oxygen 368
Sulfur 11
Total number of atoms 3684
Formula = C1153H1807N345O368S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 23755 M-1 cm-1
assuming NO Cys residues appear as half cystines 23380 M-1 cm-1
assuming all Cys residues are reduced 23380 M-1 cm-1
assuming all pairs of Cys residues form cystines 23755 M-1 cm-1

Aliphatic index
Aliphatic index 56.97

Coding Region Translation Calculations
Codon Bias -0.061
Codon Adaptation Index 0.106
Frequency of Optimal Codons 0.376
Hydropathicity of Protein -1.026
Aromaticity Score 0.087