Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
79 |
7.2% |
| Arg[R] |
39 |
3.6% |
| Asn[N] |
61 |
5.6% |
| Asp[D] |
70 |
6.4% |
| Cys[C] |
16 |
1.5% |
| Gln[Q] |
28 |
2.6% |
| Glu[E] |
60 |
5.5% |
| Gly[G] |
74 |
6.8% |
| His[H] |
22 |
2.0% |
| Ile[I] |
83 |
7.6% |
| Leu[L] |
89 |
8.2% |
| Lys[K] |
63 |
5.8% |
| Met[M] |
30 |
2.7% |
| Phe[F] |
53 |
4.9% |
| Pro[P] |
38 |
3.5% |
| Ser[S] |
88 |
8.1% |
| Thr[T] |
72 |
6.6% |
| Trp[W] |
16 |
1.5% |
| Tyr[Y] |
25 |
2.3% |
| Val[V] |
85 |
7.8% |
| Total Length |
1091 |
|
| Instability index (II) |
34.41 |
| This classifies the protein as stable |
|
| Carbon |
5368 |
| Hydrogen |
8436 |
| Nitrogen |
1420 |
| Oxygen |
1626 |
| Sulfur |
46 |
| Total number of atoms |
16896 |
| Formula = C5368H8436N1420O1626S46 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126250 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125250 M-1 cm-1 |
| assuming all Cys residues are reduced |
125250 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126250 M-1 cm-1 |
|
|
| Codon Bias |
0.193 |
| Codon Adaptation Index |
0.197 |
| Frequency of Optimal Codons |
0.526 |
| Hydropathicity of Protein |
-0.055 |
| Aromaticity Score |
0.086 |
|
