Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
80 |
7.3% |
| Arg[R] |
41 |
3.8% |
| Asn[N] |
61 |
5.6% |
| Asp[D] |
70 |
6.4% |
| Cys[C] |
16 |
1.5% |
| Gln[Q] |
28 |
2.6% |
| Glu[E] |
61 |
5.6% |
| Gly[G] |
73 |
6.7% |
| His[H] |
20 |
1.8% |
| Ile[I] |
82 |
7.5% |
| Leu[L] |
89 |
8.2% |
| Lys[K] |
63 |
5.8% |
| Met[M] |
29 |
2.7% |
| Phe[F] |
52 |
4.8% |
| Pro[P] |
38 |
3.5% |
| Ser[S] |
90 |
8.2% |
| Thr[T] |
70 |
6.4% |
| Trp[W] |
16 |
1.5% |
| Tyr[Y] |
25 |
2.3% |
| Val[V] |
87 |
8.0% |
| Total Length |
1091 |
|
| Instability index (II) |
35.67 |
| This classifies the protein as stable |
|
| Carbon |
5362 |
| Hydrogen |
8440 |
| Nitrogen |
1422 |
| Oxygen |
1628 |
| Sulfur |
45 |
| Total number of atoms |
16897 |
| Formula = C5362H8440N1422O1628S45 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126250 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125250 M-1 cm-1 |
| assuming all Cys residues are reduced |
125250 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126250 M-1 cm-1 |
|
|
| Codon Bias |
0.189 |
| Codon Adaptation Index |
0.190 |
| Frequency of Optimal Codons |
0.523 |
| Hydropathicity of Protein |
-0.059 |
| Aromaticity Score |
0.085 |
|
