Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
4.3% |
| Arg[R] |
54 |
5.1% |
| Asn[N] |
84 |
8.0% |
| Asp[D] |
76 |
7.2% |
| Cys[C] |
16 |
1.5% |
| Gln[Q] |
41 |
3.9% |
| Glu[E] |
75 |
7.1% |
| Gly[G] |
45 |
4.3% |
| His[H] |
38 |
3.6% |
| Ile[I] |
54 |
5.1% |
| Leu[L] |
85 |
8.1% |
| Lys[K] |
84 |
8.0% |
| Met[M] |
20 |
1.9% |
| Phe[F] |
37 |
3.5% |
| Pro[P] |
44 |
4.2% |
| Ser[S] |
112 |
10.7% |
| Thr[T] |
58 |
5.5% |
| Trp[W] |
8 |
0.8% |
| Tyr[Y] |
30 |
2.9% |
| Val[V] |
44 |
4.2% |
| Total Length |
1050 |
|
| Instability index (II) |
49.28 |
| This classifies the protein as unstable |
|
| Carbon |
5182 |
| Hydrogen |
8163 |
| Nitrogen |
1505 |
| Oxygen |
1678 |
| Sulfur |
36 |
| Total number of atoms |
16564 |
| Formula = C5182H8163N1505O1678S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
89700 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
88700 M-1 cm-1 |
| assuming all Cys residues are reduced |
88700 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
89700 M-1 cm-1 |
|
|
| Codon Bias |
0.016 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.422 |
| Hydropathicity of Protein |
-0.866 |
| Aromaticity Score |
0.071 |
|
