Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
20 |
3.1% |
| Arg[R] |
26 |
4.0% |
| Asn[N] |
53 |
8.2% |
| Asp[D] |
53 |
8.2% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
18 |
2.8% |
| Glu[E] |
47 |
7.3% |
| Gly[G] |
20 |
3.1% |
| His[H] |
13 |
2.0% |
| Ile[I] |
55 |
8.5% |
| Leu[L] |
72 |
11.1% |
| Lys[K] |
60 |
9.3% |
| Met[M] |
19 |
2.9% |
| Phe[F] |
35 |
5.4% |
| Pro[P] |
17 |
2.6% |
| Ser[S] |
70 |
10.8% |
| Thr[T] |
29 |
4.5% |
| Trp[W] |
3 |
0.5% |
| Tyr[Y] |
18 |
2.8% |
| Val[V] |
16 |
2.5% |
| Total Length |
647 |
|
| Instability index (II) |
47.27 |
| This classifies the protein as unstable |
|
| Carbon |
3310 |
| Hydrogen |
5247 |
| Nitrogen |
885 |
| Oxygen |
1036 |
| Sulfur |
22 |
| Total number of atoms |
10500 |
| Formula = C3310H5247N885O1036S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
43445 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
43320 M-1 cm-1 |
| assuming all Cys residues are reduced |
43320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
43445 M-1 cm-1 |
|
|
| Codon Bias |
-0.044 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.395 |
| Hydropathicity of Protein |
-0.561 |
| Aromaticity Score |
0.087 |
|
