HXT15/YDL245C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 39 6.9%
Arg[R] 17 3.0%
Asn[N] 19 3.4%
Asp[D] 14 2.5%
Cys[C] 14 2.5%
Gln[Q] 14 2.5%
Glu[E] 28 4.9%
Gly[G] 52 9.2%
His[H] 6 1.1%
Ile[I] 43 7.6%
Leu[L] 52 9.2%
Lys[K] 30 5.3%
Met[M] 16 2.8%
Phe[F] 42 7.4%
Pro[P] 24 4.2%
Ser[S] 46 8.1%
Thr[T] 26 4.6%
Trp[W] 13 2.3%
Tyr[Y] 26 4.6%
Val[V] 46 8.1%
Total Length 567

Instability index
Instability index (II) 34.87
This classifies the protein as stable

Atomic composition
Carbon 2920
Hydrogen 4446
Nitrogen 706
Oxygen 783
Sulfur 30
Total number of atoms 8885
Formula = C2920H4446N706O783S30

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 111115 M-1 cm-1
assuming NO Cys residues appear as half cystines 110240 M-1 cm-1
assuming all Cys residues are reduced 110240 M-1 cm-1
assuming all pairs of Cys residues form cystines 111115 M-1 cm-1

Aliphatic index
Aliphatic index 95.75

Coding Region Translation Calculations
Codon Bias 0.172
Codon Adaptation Index 0.162
Frequency of Optimal Codons 0.506
Hydropathicity of Protein 0.357
Aromaticity Score 0.143