Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
2.9% |
| Arg[R] |
28 |
3.8% |
| Asn[N] |
59 |
7.9% |
| Asp[D] |
50 |
6.7% |
| Cys[C] |
9 |
1.2% |
| Gln[Q] |
43 |
5.8% |
| Glu[E] |
55 |
7.4% |
| Gly[G] |
25 |
3.4% |
| His[H] |
14 |
1.9% |
| Ile[I] |
39 |
5.2% |
| Leu[L] |
93 |
12.5% |
| Lys[K] |
48 |
6.4% |
| Met[M] |
18 |
2.4% |
| Phe[F] |
45 |
6.0% |
| Pro[P] |
28 |
3.8% |
| Ser[S] |
61 |
8.2% |
| Thr[T] |
35 |
4.7% |
| Trp[W] |
19 |
2.5% |
| Tyr[Y] |
21 |
2.8% |
| Val[V] |
34 |
4.6% |
| Total Length |
746 |
|
| Instability index (II) |
46.14 |
| This classifies the protein as unstable |
|
| Carbon |
3927 |
| Hydrogen |
6025 |
| Nitrogen |
1027 |
| Oxygen |
1176 |
| Sulfur |
27 |
| Total number of atoms |
12182 |
| Formula = C3927H6025N1027O1176S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
136290 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
135790 M-1 cm-1 |
| assuming all Cys residues are reduced |
135790 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
136290 M-1 cm-1 |
|
|
| Codon Bias |
0.087 |
| Codon Adaptation Index |
0.153 |
| Frequency of Optimal Codons |
0.463 |
| Hydropathicity of Protein |
-0.484 |
| Aromaticity Score |
0.114 |
|
