Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
26 |
4.7% |
| Arg[R] |
32 |
5.8% |
| Asn[N] |
41 |
7.4% |
| Asp[D] |
29 |
5.3% |
| Cys[C] |
2 |
0.4% |
| Gln[Q] |
25 |
4.5% |
| Glu[E] |
55 |
10.0% |
| Gly[G] |
24 |
4.4% |
| His[H] |
9 |
1.6% |
| Ile[I] |
36 |
6.5% |
| Leu[L] |
49 |
8.9% |
| Lys[K] |
38 |
6.9% |
| Met[M] |
10 |
1.8% |
| Phe[F] |
21 |
3.8% |
| Pro[P] |
25 |
4.5% |
| Ser[S] |
54 |
9.8% |
| Thr[T] |
34 |
6.2% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
16 |
2.9% |
| Val[V] |
24 |
4.4% |
| Total Length |
551 |
|
| Instability index (II) |
42.84 |
| This classifies the protein as unstable |
|
| Carbon |
2733 |
| Hydrogen |
4360 |
| Nitrogen |
770 |
| Oxygen |
890 |
| Sulfur |
12 |
| Total number of atoms |
8765 |
| Formula = C2733H4360N770O890S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
29465 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
29340 M-1 cm-1 |
| assuming all Cys residues are reduced |
29340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
29465 M-1 cm-1 |
|
|
| Codon Bias |
0.184 |
| Codon Adaptation Index |
0.190 |
| Frequency of Optimal Codons |
0.519 |
| Hydropathicity of Protein |
-0.736 |
| Aromaticity Score |
0.069 |
|
