Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
57 |
6.4% |
| Arg[R] |
34 |
3.8% |
| Asn[N] |
57 |
6.4% |
| Asp[D] |
47 |
5.3% |
| Cys[C] |
7 |
0.8% |
| Gln[Q] |
32 |
3.6% |
| Glu[E] |
42 |
4.8% |
| Gly[G] |
64 |
7.2% |
| His[H] |
14 |
1.6% |
| Ile[I] |
66 |
7.5% |
| Leu[L] |
69 |
7.8% |
| Lys[K] |
34 |
3.8% |
| Met[M] |
20 |
2.3% |
| Phe[F] |
48 |
5.4% |
| Pro[P] |
35 |
4.0% |
| Ser[S] |
101 |
11.4% |
| Thr[T] |
64 |
7.2% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
28 |
3.2% |
| Val[V] |
58 |
6.6% |
| Total Length |
884 |
|
| Instability index (II) |
39.82 |
| This classifies the protein as stable |
|
| Carbon |
4293 |
| Hydrogen |
6694 |
| Nitrogen |
1144 |
| Oxygen |
1345 |
| Sulfur |
27 |
| Total number of atoms |
13503 |
| Formula = C4293H6694N1144O1345S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
80595 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
80220 M-1 cm-1 |
| assuming all Cys residues are reduced |
80220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
80595 M-1 cm-1 |
|
|
| Codon Bias |
0.066 |
| Codon Adaptation Index |
0.130 |
| Frequency of Optimal Codons |
0.447 |
| Hydropathicity of Protein |
-0.122 |
| Aromaticity Score |
0.094 |
|
