Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
80 |
7.5% |
| Arg[R] |
55 |
5.1% |
| Asn[N] |
38 |
3.5% |
| Asp[D] |
61 |
5.7% |
| Cys[C] |
12 |
1.1% |
| Gln[Q] |
27 |
2.5% |
| Glu[E] |
81 |
7.6% |
| Gly[G] |
83 |
7.7% |
| His[H] |
20 |
1.9% |
| Ile[I] |
63 |
5.9% |
| Leu[L] |
87 |
8.1% |
| Lys[K] |
71 |
6.6% |
| Met[M] |
23 |
2.1% |
| Phe[F] |
41 |
3.8% |
| Pro[P] |
45 |
4.2% |
| Ser[S] |
87 |
8.1% |
| Thr[T] |
64 |
6.0% |
| Trp[W] |
10 |
0.9% |
| Tyr[Y] |
43 |
4.0% |
| Val[V] |
80 |
7.5% |
| Total Length |
1071 |
|
| Instability index (II) |
33.01 |
| This classifies the protein as stable |
|
| Carbon |
5277 |
| Hydrogen |
8310 |
| Nitrogen |
1422 |
| Oxygen |
1615 |
| Sulfur |
35 |
| Total number of atoms |
16659 |
| Formula = C5277H8310N1422O1615S35 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
119820 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
119070 M-1 cm-1 |
| assuming all Cys residues are reduced |
119070 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
119820 M-1 cm-1 |
|
|
| Codon Bias |
0.357 |
| Codon Adaptation Index |
0.305 |
| Frequency of Optimal Codons |
0.615 |
| Hydropathicity of Protein |
-0.294 |
| Aromaticity Score |
0.088 |
|
