INH1/YDL181W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 5.9%
Arg[R] 11 12.9%
Asn[N] 1 1.2%
Asp[D] 4 4.7%
Cys[C] 0 0.0%
Gln[Q] 6 7.1%
Glu[E] 9 10.6%
Gly[G] 5 5.9%
His[H] 1 1.2%
Ile[I] 2 2.4%
Leu[L] 8 9.4%
Lys[K] 8 9.4%
Met[M] 2 2.4%
Phe[F] 4 4.7%
Pro[P] 2 2.4%
Ser[S] 9 10.6%
Thr[T] 4 4.7%
Trp[W] 0 0.0%
Tyr[Y] 1 1.2%
Val[V] 3 3.5%
Total Length 85

Instability index
Instability index (II) 62.53
This classifies the protein as unstable

Atomic composition
Carbon 423
Hydrogen 701
Nitrogen 135
Oxygen 133
Sulfur 2
Total number of atoms 1394
Formula = C423H701N135O133S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 1490 M-1 cm-1

Aliphatic index
Aliphatic index 62.00

Coding Region Translation Calculations
Codon Bias 0.115
Codon Adaptation Index 0.130
Frequency of Optimal Codons 0.446
Hydropathicity of Protein -1.111
Aromaticity Score 0.059