YDL172C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 11 6.9%
Arg[R] 2 1.3%
Asn[N] 5 3.1%
Asp[D] 6 3.8%
Cys[C] 1 0.6%
Gln[Q] 1 0.6%
Glu[E] 4 2.5%
Gly[G] 5 3.1%
His[H] 0 0.0%
Ile[I] 9 5.7%
Leu[L] 31 19.5%
Lys[K] 8 5.0%
Met[M] 3 1.9%
Phe[F] 24 15.1%
Pro[P] 16 10.1%
Ser[S] 20 12.6%
Thr[T] 5 3.1%
Trp[W] 1 0.6%
Tyr[Y] 0 0.0%
Val[V] 7 4.4%
Total Length 159

Instability index
Instability index (II) 44.54
This classifies the protein as unstable

Atomic composition
Carbon 852
Hydrogen 1296
Nitrogen 180
Oxygen 211
Sulfur 4
Total number of atoms 2543
Formula = C852H1296N180O211S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 5500 M-1 cm-1
assuming NO Cys residues appear as half cystines 5500 M-1 cm-1
assuming all Cys residues are reduced 5500 M-1 cm-1
assuming all pairs of Cys residues form cystines 5500 M-1 cm-1

Aliphatic index
Aliphatic index 117.80

Coding Region Translation Calculations
Codon Bias 0.156
Codon Adaptation Index 0.141
Frequency of Optimal Codons 0.465
Hydropathicity of Protein 0.872
Aromaticity Score 0.157