CDC9/YDL164C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 37 4.9%
Arg[R] 37 4.9%
Asn[N] 33 4.4%
Asp[D] 46 6.1%
Cys[C] 16 2.1%
Gln[Q] 26 3.4%
Glu[E] 55 7.3%
Gly[G] 45 6.0%
His[H] 10 1.3%
Ile[I] 42 5.6%
Leu[L] 83 11.0%
Lys[K] 65 8.6%
Met[M] 22 2.9%
Phe[F] 29 3.8%
Pro[P] 35 4.6%
Ser[S] 65 8.6%
Thr[T] 46 6.1%
Trp[W] 3 0.4%
Tyr[Y] 23 3.0%
Val[V] 37 4.9%
Total Length 755

Instability index
Instability index (II) 40.42
This classifies the protein as unstable

Atomic composition
Carbon 3742
Hydrogen 6013
Nitrogen 1013
Oxygen 1151
Sulfur 38
Total number of atoms 11957
Formula = C3742H6013N1013O1151S38

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 51770 M-1 cm-1
assuming NO Cys residues appear as half cystines 50770 M-1 cm-1
assuming all Cys residues are reduced 50770 M-1 cm-1
assuming all pairs of Cys residues form cystines 51770 M-1 cm-1

Aliphatic index
Aliphatic index 83.68

Coding Region Translation Calculations
Codon Bias 0.139
Codon Adaptation Index 0.160
Frequency of Optimal Codons 0.484
Hydropathicity of Protein -0.415
Aromaticity Score 0.073