Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
9 |
4.0% |
| Arg[R] |
13 |
5.8% |
| Asn[N] |
14 |
6.3% |
| Asp[D] |
25 |
11.2% |
| Cys[C] |
1 |
0.4% |
| Gln[Q] |
1 |
0.4% |
| Glu[E] |
23 |
10.3% |
| Gly[G] |
9 |
4.0% |
| His[H] |
7 |
3.1% |
| Ile[I] |
18 |
8.1% |
| Leu[L] |
15 |
6.7% |
| Lys[K] |
32 |
14.3% |
| Met[M] |
4 |
1.8% |
| Phe[F] |
1 |
0.4% |
| Pro[P] |
5 |
2.2% |
| Ser[S] |
18 |
8.1% |
| Thr[T] |
11 |
4.9% |
| Trp[W] |
2 |
0.9% |
| Tyr[Y] |
6 |
2.7% |
| Val[V] |
9 |
4.0% |
| Total Length |
223 |
|
| Instability index (II) |
44.41 |
| This classifies the protein as unstable |
|
| Carbon |
1107 |
| Hydrogen |
1811 |
| Nitrogen |
325 |
| Oxygen |
370 |
| Sulfur |
5 |
| Total number of atoms |
3618 |
| Formula = C1107H1811N325O370S5 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
19940 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
19940 M-1 cm-1 |
| assuming all Cys residues are reduced |
19940 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
19940 M-1 cm-1 |
|
|
| Codon Bias |
-0.020 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-1.187 |
| Aromaticity Score |
0.040 |
|
