POL3/YDL102W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 58 5.3%
Arg[R] 56 5.1%
Asn[N] 62 5.7%
Asp[D] 70 6.4%
Cys[C] 20 1.8%
Gln[Q] 40 3.6%
Glu[E] 64 5.8%
Gly[G] 59 5.4%
His[H] 19 1.7%
Ile[I] 79 7.2%
Leu[L] 96 8.8%
Lys[K] 84 7.7%
Met[M] 26 2.4%
Phe[F] 44 4.0%
Pro[P] 44 4.0%
Ser[S] 79 7.2%
Thr[T] 60 5.5%
Trp[W] 9 0.8%
Tyr[Y] 49 4.5%
Val[V] 79 7.2%
Total Length 1097

Instability index
Instability index (II) 38.27
This classifies the protein as stable

Atomic composition
Carbon 5562
Hydrogen 8792
Nitrogen 1498
Oxygen 1656
Sulfur 46
Total number of atoms 17554
Formula = C5562H8792N1498O1656S46

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 123760 M-1 cm-1
assuming NO Cys residues appear as half cystines 122510 M-1 cm-1
assuming all Cys residues are reduced 122510 M-1 cm-1
assuming all pairs of Cys residues form cystines 123760 M-1 cm-1

Aliphatic index
Aliphatic index 88.39

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000