Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
56 |
6.9% |
| Arg[R] |
27 |
3.3% |
| Asn[N] |
30 |
3.7% |
| Asp[D] |
36 |
4.4% |
| Cys[C] |
9 |
1.1% |
| Gln[Q] |
25 |
3.1% |
| Glu[E] |
45 |
5.5% |
| Gly[G] |
51 |
6.2% |
| His[H] |
25 |
3.1% |
| Ile[I] |
43 |
5.3% |
| Leu[L] |
84 |
10.3% |
| Lys[K] |
44 |
5.4% |
| Met[M] |
22 |
2.7% |
| Phe[F] |
52 |
6.4% |
| Pro[P] |
36 |
4.4% |
| Ser[S] |
68 |
8.3% |
| Thr[T] |
43 |
5.3% |
| Trp[W] |
20 |
2.4% |
| Tyr[Y] |
40 |
4.9% |
| Val[V] |
61 |
7.5% |
| Total Length |
817 |
|
| Instability index (II) |
42.64 |
| This classifies the protein as unstable |
|
| Carbon |
4268 |
| Hydrogen |
6447 |
| Nitrogen |
1067 |
| Oxygen |
1186 |
| Sulfur |
31 |
| Total number of atoms |
12999 |
| Formula = C4268H6447N1067O1186S31 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
170100 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
169600 M-1 cm-1 |
| assuming all Cys residues are reduced |
169600 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
170100 M-1 cm-1 |
|
|
| Codon Bias |
0.268 |
| Codon Adaptation Index |
0.227 |
| Frequency of Optimal Codons |
0.561 |
| Hydropathicity of Protein |
-0.002 |
| Aromaticity Score |
0.137 |
|
