YDL094C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 3.6%
Arg[R] 9 5.3%
Asn[N] 7 4.1%
Asp[D] 7 4.1%
Cys[C] 3 1.8%
Gln[Q] 5 3.0%
Glu[E] 7 4.1%
Gly[G] 6 3.6%
His[H] 5 3.0%
Ile[I] 13 7.7%
Leu[L] 17 10.1%
Lys[K] 14 8.3%
Met[M] 7 4.1%
Phe[F] 16 9.5%
Pro[P] 4 2.4%
Ser[S] 18 10.7%
Thr[T] 6 3.6%
Trp[W] 0 0.0%
Tyr[Y] 7 4.1%
Val[V] 12 7.1%
Total Length 169

Instability index
Instability index (II) 39.31
This classifies the protein as stable

Atomic composition
Carbon 903
Hydrogen 1410
Nitrogen 232
Oxygen 241
Sulfur 10
Total number of atoms 2796
Formula = C903H1410N232O241S10

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10555 M-1 cm-1
assuming NO Cys residues appear as half cystines 10430 M-1 cm-1
assuming all Cys residues are reduced 10430 M-1 cm-1
assuming all pairs of Cys residues form cystines 10555 M-1 cm-1

Aliphatic index
Aliphatic index 93.37

Coding Region Translation Calculations
Codon Bias -0.069
Codon Adaptation Index 0.114
Frequency of Optimal Codons 0.377
Hydropathicity of Protein 0.067
Aromaticity Score 0.136