Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.6% |
| Arg[R] |
37 |
5.3% |
| Asn[N] |
43 |
6.1% |
| Asp[D] |
49 |
7.0% |
| Cys[C] |
13 |
1.9% |
| Gln[Q] |
29 |
4.1% |
| Glu[E] |
78 |
11.1% |
| Gly[G] |
10 |
1.4% |
| His[H] |
13 |
1.9% |
| Ile[I] |
46 |
6.6% |
| Leu[L] |
80 |
11.4% |
| Lys[K] |
82 |
11.7% |
| Met[M] |
8 |
1.1% |
| Phe[F] |
21 |
3.0% |
| Pro[P] |
6 |
0.9% |
| Ser[S] |
69 |
9.9% |
| Thr[T] |
43 |
6.1% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
11 |
1.6% |
| Val[V] |
21 |
3.0% |
| Total Length |
700 |
|
| Instability index (II) |
44.41 |
| This classifies the protein as unstable |
|
| Carbon |
3491 |
| Hydrogen |
5735 |
| Nitrogen |
993 |
| Oxygen |
1150 |
| Sulfur |
21 |
| Total number of atoms |
11390 |
| Formula = C3491H5735N993O1150S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
28140 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
27390 M-1 cm-1 |
| assuming all Cys residues are reduced |
27390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
28140 M-1 cm-1 |
|
|
| Codon Bias |
0.011 |
| Codon Adaptation Index |
0.141 |
| Frequency of Optimal Codons |
0.425 |
| Hydropathicity of Protein |
-0.805 |
| Aromaticity Score |
0.049 |
|
