Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
30 |
7.0% |
| Arg[R] |
20 |
4.7% |
| Asn[N] |
13 |
3.0% |
| Asp[D] |
28 |
6.5% |
| Cys[C] |
3 |
0.7% |
| Gln[Q] |
15 |
3.5% |
| Glu[E] |
27 |
6.3% |
| Gly[G] |
28 |
6.5% |
| His[H] |
8 |
1.9% |
| Ile[I] |
34 |
7.9% |
| Leu[L] |
37 |
8.6% |
| Lys[K] |
41 |
9.6% |
| Met[M] |
11 |
2.6% |
| Phe[F] |
16 |
3.7% |
| Pro[P] |
16 |
3.7% |
| Ser[S] |
29 |
6.8% |
| Thr[T] |
29 |
6.8% |
| Trp[W] |
4 |
0.9% |
| Tyr[Y] |
17 |
4.0% |
| Val[V] |
22 |
5.1% |
| Total Length |
428 |
|
| Instability index (II) |
33.13 |
| This classifies the protein as stable |
|
| Carbon |
2158 |
| Hydrogen |
3441 |
| Nitrogen |
577 |
| Oxygen |
642 |
| Sulfur |
14 |
| Total number of atoms |
6832 |
| Formula = C2158H3441N577O642S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
47455 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
47330 M-1 cm-1 |
| assuming all Cys residues are reduced |
47330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
47455 M-1 cm-1 |
|
|
| Codon Bias |
0.408 |
| Codon Adaptation Index |
0.319 |
| Frequency of Optimal Codons |
0.651 |
| Hydropathicity of Protein |
-0.371 |
| Aromaticity Score |
0.086 |
|
