Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
21 |
4.8% |
| Arg[R] |
28 |
6.4% |
| Asn[N] |
40 |
9.1% |
| Asp[D] |
26 |
5.9% |
| Cys[C] |
6 |
1.4% |
| Gln[Q] |
13 |
3.0% |
| Glu[E] |
42 |
9.5% |
| Gly[G] |
13 |
3.0% |
| His[H] |
8 |
1.8% |
| Ile[I] |
29 |
6.6% |
| Leu[L] |
48 |
10.9% |
| Lys[K] |
37 |
8.4% |
| Met[M] |
9 |
2.0% |
| Phe[F] |
13 |
3.0% |
| Pro[P] |
9 |
2.0% |
| Ser[S] |
30 |
6.8% |
| Thr[T] |
19 |
4.3% |
| Trp[W] |
2 |
0.5% |
| Tyr[Y] |
19 |
4.3% |
| Val[V] |
28 |
6.4% |
| Total Length |
440 |
|
| Instability index (II) |
40.81 |
| This classifies the protein as unstable |
|
| Carbon |
2252 |
| Hydrogen |
3614 |
| Nitrogen |
632 |
| Oxygen |
698 |
| Sulfur |
15 |
| Total number of atoms |
7211 |
| Formula = C2252H3614N632O698S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
39685 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
39310 M-1 cm-1 |
| assuming all Cys residues are reduced |
39310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
39685 M-1 cm-1 |
|
|
| Codon Bias |
0.107 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.478 |
| Hydropathicity of Protein |
-0.605 |
| Aromaticity Score |
0.077 |
|
