GPR1/YDL035C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 45 4.7%
Arg[R] 36 3.7%
Asn[N] 125 13.0%
Asp[D] 58 6.0%
Cys[C] 11 1.1%
Gln[Q] 26 2.7%
Glu[E] 49 5.1%
Gly[G] 41 4.3%
His[H] 17 1.8%
Ile[I] 72 7.5%
Leu[L] 86 8.9%
Lys[K] 68 7.1%
Met[M] 18 1.9%
Phe[F] 55 5.7%
Pro[P] 42 4.4%
Ser[S] 80 8.3%
Thr[T] 45 4.7%
Trp[W] 21 2.2%
Tyr[Y] 37 3.9%
Val[V] 29 3.0%
Total Length 961

Instability index
Instability index (II) 38.23
This classifies the protein as stable

Atomic composition
Carbon 4955
Hydrogen 7571
Nitrogen 1343
Oxygen 1489
Sulfur 29
Total number of atoms 15387
Formula = C4955H7571N1343O1489S29

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 171255 M-1 cm-1
assuming NO Cys residues appear as half cystines 170630 M-1 cm-1
assuming all Cys residues are reduced 170630 M-1 cm-1
assuming all pairs of Cys residues form cystines 171255 M-1 cm-1

Aliphatic index
Aliphatic index 77.55

Coding Region Translation Calculations
Codon Bias -0.043
Codon Adaptation Index 0.122
Frequency of Optimal Codons 0.395
Hydropathicity of Protein -0.584
Aromaticity Score 0.118