Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
6.1% |
| Arg[R] |
73 |
11.8% |
| Asn[N] |
36 |
5.8% |
| Asp[D] |
50 |
8.1% |
| Cys[C] |
10 |
1.6% |
| Gln[Q] |
26 |
4.2% |
| Glu[E] |
53 |
8.6% |
| Gly[G] |
30 |
4.8% |
| His[H] |
12 |
1.9% |
| Ile[I] |
35 |
5.7% |
| Leu[L] |
48 |
7.8% |
| Lys[K] |
25 |
4.0% |
| Met[M] |
10 |
1.6% |
| Phe[F] |
23 |
3.7% |
| Pro[P] |
26 |
4.2% |
| Ser[S] |
47 |
7.6% |
| Thr[T] |
40 |
6.5% |
| Trp[W] |
3 |
0.5% |
| Tyr[Y] |
10 |
1.6% |
| Val[V] |
24 |
3.9% |
| Total Length |
619 |
|
| Instability index (II) |
52.51 |
| This classifies the protein as unstable |
|
| Carbon |
3032 |
| Hydrogen |
4880 |
| Nitrogen |
952 |
| Oxygen |
985 |
| Sulfur |
20 |
| Total number of atoms |
9869 |
| Formula = C3032H4880N952O985S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
32025 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
31400 M-1 cm-1 |
| assuming all Cys residues are reduced |
31400 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
32025 M-1 cm-1 |
|
|
| Codon Bias |
0.013 |
| Codon Adaptation Index |
0.112 |
| Frequency of Optimal Codons |
0.399 |
| Hydropathicity of Protein |
-0.904 |
| Aromaticity Score |
0.058 |
|
