MCD1/YDL003W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 31 5.5%
Arg[R] 27 4.8%
Asn[N] 41 7.2%
Asp[D] 42 7.4%
Cys[C] 2 0.4%
Gln[Q] 22 3.9%
Glu[E] 58 10.2%
Gly[G] 29 5.1%
His[H] 5 0.9%
Ile[I] 40 7.1%
Leu[L] 47 8.3%
Lys[K] 31 5.5%
Met[M] 13 2.3%
Phe[F] 21 3.7%
Pro[P] 24 4.2%
Ser[S] 47 8.3%
Thr[T] 52 9.2%
Trp[W] 5 0.9%
Tyr[Y] 6 1.1%
Val[V] 23 4.1%
Total Length 566

Instability index
Instability index (II) 50.01
This classifies the protein as unstable

Atomic composition
Carbon 2736
Hydrogen 4364
Nitrogen 756
Oxygen 935
Sulfur 15
Total number of atoms 8806
Formula = C2736H4364N756O935S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 36565 M-1 cm-1
assuming NO Cys residues appear as half cystines 36440 M-1 cm-1
assuming all Cys residues are reduced 36440 M-1 cm-1
assuming all pairs of Cys residues form cystines 36565 M-1 cm-1

Aliphatic index
Aliphatic index 77.21

Coding Region Translation Calculations
Codon Bias 0.043
Codon Adaptation Index 0.136
Frequency of Optimal Codons 0.440
Hydropathicity of Protein -0.646
Aromaticity Score 0.057