NHP6B/YBR089C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 12.1%
Arg[R] 10 10.1%
Asn[N] 5 5.1%
Asp[D] 4 4.0%
Cys[C] 0 0.0%
Gln[Q] 4 4.0%
Glu[E] 11 11.1%
Gly[G] 4 4.0%
His[H] 0 0.0%
Ile[I] 2 2.0%
Leu[L] 4 4.0%
Lys[K] 14 14.1%
Met[M] 2 2.0%
Phe[F] 3 3.0%
Pro[P] 6 6.1%
Ser[S] 4 4.0%
Thr[T] 6 6.1%
Trp[W] 1 1.0%
Tyr[Y] 4 4.0%
Val[V] 3 3.0%
Total Length 99

Instability index
Instability index (II) 58.30
This classifies the protein as unstable

Atomic composition
Carbon 500
Hydrogen 809
Nitrogen 153
Oxygen 153
Sulfur 2
Total number of atoms 1617
Formula = C500H809N153O153S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 11460 M-1 cm-1

Aliphatic index
Aliphatic index 44.55

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000