YBR012W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 80 4.6%
Arg[R] 73 4.2%
Asn[N] 124 7.1%
Asp[D] 106 6.0%
Cys[C] 15 0.9%
Gln[Q] 75 4.3%
Glu[E] 83 4.7%
Gly[G] 68 3.9%
His[H] 60 3.4%
Ile[I] 127 7.2%
Leu[L] 145 8.3%
Lys[K] 129 7.3%
Met[M] 32 1.8%
Phe[F] 53 3.0%
Pro[P] 111 6.3%
Ser[S] 179 10.2%
Thr[T] 132 7.5%
Trp[W] 14 0.8%
Tyr[Y] 77 4.4%
Val[V] 73 4.2%
Total Length 1756

Instability index
Instability index (II) 46.25
This classifies the protein as unstable

Atomic composition
Carbon 8804
Hydrogen 13823
Nitrogen 2437
Oxygen 2722
Sulfur 47
Total number of atoms 27833
Formula = C8804H13823N2437O2722S47

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 192605 M-1 cm-1
assuming NO Cys residues appear as half cystines 191730 M-1 cm-1
assuming all Cys residues are reduced 191730 M-1 cm-1
assuming all pairs of Cys residues form cystines 192605 M-1 cm-1

Aliphatic index
Aliphatic index 77.02

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000