FUN19/YAL034C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 21 5.1%
Arg[R] 28 6.8%
Asn[N] 40 9.7%
Asp[D] 20 4.8%
Cys[C] 3 0.7%
Gln[Q] 15 3.6%
Glu[E] 13 3.1%
Gly[G] 16 3.9%
His[H] 7 1.7%
Ile[I] 17 4.1%
Leu[L] 40 9.7%
Lys[K] 32 7.7%
Met[M] 8 1.9%
Phe[F] 12 2.9%
Pro[P] 34 8.2%
Ser[S] 51 12.3%
Thr[T] 15 3.6%
Trp[W] 5 1.2%
Tyr[Y] 22 5.3%
Val[V] 14 3.4%
Total Length 413

Instability index
Instability index (II) 57.07
This classifies the protein as unstable

Atomic composition
Carbon 2082
Hydrogen 3269
Nitrogen 603
Oxygen 623
Sulfur 11
Total number of atoms 6588
Formula = C2082H3269N603O623S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 60405 M-1 cm-1
assuming NO Cys residues appear as half cystines 60280 M-1 cm-1
assuming all Cys residues are reduced 60280 M-1 cm-1
assuming all pairs of Cys residues form cystines 60405 M-1 cm-1

Aliphatic index
Aliphatic index 68.74

Coding Region Translation Calculations
Codon Bias 0.073
Codon Adaptation Index 0.125
Frequency of Optimal Codons 0.430
Hydropathicity of Protein -0.835
Aromaticity Score 0.094