VMA10/YHR039C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 14 12.3%
Arg[R] 2 1.8%
Asn[N] 3 2.6%
Asp[D] 6 5.3%
Cys[C] 0 0.0%
Gln[Q] 7 6.1%
Glu[E] 15 13.2%
Gly[G] 6 5.3%
His[H] 2 1.8%
Ile[I] 10 8.8%
Leu[L] 8 7.0%
Lys[K] 22 19.3%
Met[M] 1 0.9%
Phe[F] 1 0.9%
Pro[P] 1 0.9%
Ser[S] 4 3.5%
Thr[T] 3 2.6%
Trp[W] 0 0.0%
Tyr[Y] 2 1.8%
Val[V] 7 6.1%
Total Length 114

Instability index
Instability index (II) 28.92
This classifies the protein as stable

Atomic composition
Carbon 560
Hydrogen 946
Nitrogen 156
Oxygen 176
Sulfur 1
Total number of atoms 1839
Formula = C560H946N156O176S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 91.67

Coding Region Translation Calculations
Codon Bias 0.407
Codon Adaptation Index 0.384
Frequency of Optimal Codons 0.664
Hydropathicity of Protein -0.762
Aromaticity Score 0.026