PRE4/YFR050C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 23 8.6%
Arg[R] 13 4.9%
Asn[N] 20 7.5%
Asp[D] 17 6.4%
Cys[C] 0 0.0%
Gln[Q] 10 3.8%
Glu[E] 11 4.1%
Gly[G] 19 7.1%
His[H] 3 1.1%
Ile[I] 18 6.8%
Leu[L] 21 7.9%
Lys[K] 12 4.5%
Met[M] 9 3.4%
Phe[F] 8 3.0%
Pro[P] 11 4.1%
Ser[S] 18 6.8%
Thr[T] 16 6.0%
Trp[W] 3 1.1%
Tyr[Y] 13 4.9%
Val[V] 21 7.9%
Total Length 266

Instability index
Instability index (II) 27.24
This classifies the protein as stable

Atomic composition
Carbon 1307
Hydrogen 2054
Nitrogen 356
Oxygen 400
Sulfur 9
Total number of atoms 4126
Formula = C1307H2054N356O400S9

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 35870 M-1 cm-1

Aliphatic index
Aliphatic index 88.72

Coding Region Translation Calculations
Codon Bias 0.109
Codon Adaptation Index 0.148
Frequency of Optimal Codons 0.469
Hydropathicity of Protein -0.220
Aromaticity Score 0.090